Abstract Light scattering serves as a non-invasive tool for monitoring structural deformities at tissue level by utilizing the change in refractive index (tissue optical property), without requiring dyes or labels. Using computerized analysis of polarized microscopy images, this study aimed at quantifying the scattering intensities in the plant specimen Alternanthera philoxeroides under normal, dehydrated, and rehydrated states to identify localized, real-time responses to hydration changes. The study showed that scattering intensity may serve as a potential non-destructive, real-time biomarker for plant tissue hydration and structural integrity under stress, using Alternanthera philoxeroides as a model. Keywords: Image Analysis, Tissue Optical Scattering, Polarized Microscopy, Pixel Intensity Quantification, Water Stressor
Roy et al. (Tue,) studied this question.