Evaluation the Effect of Local Application of Hydroxyapatite/Beta Tricalcium Phosphate and Hyaluronic Acid on Bone Healing

Abstract Background: Bone regeneration is a complicated, well-organized physiological process of bone creation that is, visible during normal fracture healing and is involved in continual remodeling during an adult’s life. Objectives: The aim of this study was to evaluate the osteogenic potential of hydroxyapatite/beta tricalcium phosphate (HA/ β -TCP) and hyaluronic acid (HY) separately or in combination. Materials and Methods: Thirty-two males albino rats, were used in this experimental study. Intra bony defect of about (2.5 mm) in width and (2.5 mm) in depth were created in the distal side of both right and left femurs bones of each rat. Four groups of animals were created at random: The control group was left to heal naturally. Experimental group 1 received HY gel and experimental group 2 received HA/ β -TCP materials, experimental group 3 received a mixture of HA/ β -TCP and HY in the bone defect. Rats were slaughtered after 2 and 4 weeks of recovery. Histological sections were used for the histological evaluation, and Image J software was used for histomorphometry analysis for assessment of osteoclasts, osteoblasts, osteocytes, trabecular number, trabecular area, and bone marrow space area. Results: It demonstrated that bone defect dealt with HA/ β -TCP and HY demonstrated earlier mineralization, maturation, and bone formation than the control group. Conclusion: Results showed that using HA/ β -TCP and HY together appeared to be more effective in osteoconduction than using HA/ β -TCP alone.