What question did this study set out to answer?

To investigate the efficacy of cavitation-based focused ultrasound in clearing vitreous hemorrhage in a rabbit model.

May 9, 2026Open Access

Focused Ultrasound Treatment of Vitreous Hemorrhage in an In Vivo Rabbit Model

Puntos clave

To investigate the efficacy of cavitation-based focused ultrasound in clearing vitreous hemorrhage in a rabbit model.
Preclinical randomized controlled trial with ten rabbits having unilateral vitreous hemorrhage
Rabbits were assigned to focused ultrasound treatment (n=5) or sham treatment (n=5)
Clinical evaluations and assessments including indirect ophthalmoscopy and B-scan ultrasonography were performed until VH resolution, up to 3 months.
50% of FUS-treated eyes achieved complete VH clearance by week 3, all by week 6
FUS treatment significantly improved VH appearance, reducing pixel intensity by an average of 41%
No consistent damage to retinal or optic nerve tissues was observed in FUS-treated groups.

Resumen

Purpose: To investigate whether cavitation-based focused ultrasound (FUS) can safely accelerate vitreous hemorrhage (VH) clearance in an in vivo model of VH.Design: Preclinical randomized controlled trial.Subjects: New Zealand rabbits.Methods: Ten rabbits with unilateral experimental VH were randomized to receive FUS (n = 5) or sham treatment (n = 5).The fellow nontreated eye of each rabbit acted as intereye controls (n = 10).Each eye was serially examined until VH resolved, or up to a maximum of 3 months.At each follow-up, clinical examination with indirect ophthalmoscopy was performed which included clinical VH grading, fundus imaging, B-scan ultrasonography, and electroretinography (ERG).After the terminal follow-up, rabbits were sacrificed and enucleated eyes underwent histologic assessment.Main Outcome Measures: Primary outcome was time-to-VH clearance based on clinical grading.Secondary outcomes included corresponding B-scan pixel intensity and ERG measurements.Any effects of FUS on nontarget tissues were further described with clinical examination and histologic assessments on enucleated eyes.Results: Five out of 5 eyes in the FUS-treated group had an immediate modification of the VH appearance on Bscan, with an average pixel intensity decrease of the VH area by 41 10%.Kaplan-Meier analysis of VH clearance by clinical examination showed that 50% of the FUS-treated eyes reached complete VH clearance by week 3, and all cleared by week 6.Sham-treated eyes started to recover at week 10, and by week 12, probability of recovery was 75%.The difference of treatment effects between these 2 groups was significant (log rank test, P = 0.004).The averaged dark-adapted implicit time between FUS-treated, sham-treated, and control eye groups correlated with the results of the clinical grading of VH.The FUS-treated group did not exhibit any consistent evidence of retinal or optic nerve damage on clinical, ERG, and histology assessment.Conclusions: This study demonstrates that cavitation-based FUS therapy accelerates VH clearance in a preclinical model when compared with natural reabsorption ("watchful waiting").With further refinement of treatment protocol to enhance safety, these findings provide initial support for vitreosonication as a potential noninvasive treatment modality for VH.

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