Seasonal and environmental drivers of antibiotic resistance and virulence in Escherichia coli from aquaculture and their public health implications

were widely distributed, particularly during warmer months and at neutral pH levels. Groups with elevated water temperatures exhibited a higher prevalence of antibiotic-resistant isolates, often harboring multiple resistance genes. This study highlights the significant role of environmental stressors in influencing the prevalence, pathogenicity, and antibiotic resistance profiles of E. coli in aquaculture systems. The findings emphasize the need for continuous monitoring and improved biosecurity measures to mitigate the risks associated with MDR E. coli in aquaculture, ensuring food safety and protecting public health.