January 1, 1987Open Access

Electroporation for the efficient transfection of mammalian cells with DNA

Puntos clave

Los puntos clave no están disponibles para este artículo en este momento.

Resumen

A simple and reproducible procedure for the introduction of DNA into mammalian cells by electroporation is described. The parameters involving the cells, the DNA, and the electric field are investigated. The procedure has been applied to a broad range of animal cells. It is capable of transforming more than 1% of the viable cells to the stable expression of a selectable marker.

Leer artículo completoexternamente

Me gusta

Guardar

Ver artículo completo

Cite This Study

Chu et al. (Thu,) studied this question.

synapsesocial.com/papers/6a09f50516dfdfe7ed348119 https://doi.org/https://doi.org/10.1093/nar/15.3.1311

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Isolation of UV-resistant revertants from a xeroderma pigmentosum complementation group A cell line1984 · 55 citations
2High efficiency polyoma DNA transfection of chloroquine treated cells1983 · 714 citations
3A Vector That Replicates as a Plasmid and Can Be Efficiently Selected in B-Lymphoblasts Transformed by Epstein-Barr Virus1985 · 189 citations
4Enhancer-dependent expression of human kappa immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation.1984 · 885 citations
5Gene transfer into mouse lyoma cells by electroporation in high electric fields.1982 · 2,475 citations

Me gusta

Guardar

Ver artículo completo