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November 21, 2025Scientific ReportsOpen Access

Upregulated BCL3, MMP9, NAMPT, and TLR4 linked to cellular senescence and demethylation in AMI.

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Why the study?

Although AMI triggers cardiomyocyte senescence and alters methylation modifications, the association between cellular senescence and demethylation remained unclear.

Population

AMI and control samples from public databases

Comparison

AMI vs control samples

Design

Bioinformatics and machine learning analysis with RT-qPCR validation

Key result

Bioinformatics analysis and experimental validation identified BCL3, MMP9, NAMPT, and TLR4 as significantly upregulated key biomarkers associated with cellular senescence and demethylation in acute myocardial infarction.

Authors

WSWenhua SuHHHong HuangQRQingrong Ruan

Discussion

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Member takes

Overview

These senescence biomarkers merit prospective validation in AMI; leaves open their prognostic or therapeutic utility.

Study Design

Type

Observational (n=133)

Multicenter

No

PICO

P
Population
Acute myocardial infarction (n=133)
I
Intervention / Comparator
Biomarker identification (BCL3, MMP9, NAMPT, TLR4) vs Healthy controls
O
Primary Outcome
Differential expression of BCL3, MMP9, NAMPT, and TLR4, p=<0.05

Main Result

p-value: p=<0.05

Limitations

  • Analysis based on public databases may be affected by batch effects and lacks independent validation for different AMI subtypes.
  • Direct regulatory effects of key genes on downstream pathways have not yet been experimentally validated.
  • Drug prediction results require further in vivo and in vitro experimental validation.

Cite This Study

Su et al. (2025) conducted an observational in Acute myocardial infarction (n=133). Biomarker identification (BCL3, MMP9, NAMPT, TLR4) vs. Healthy controls was evaluated on Differential expression of BCL3, MMP9, NAMPT, and TLR4 (p=<0.05). Bioinformatics analysis and experimental validation identified BCL3, MMP9, NAMPT, and TLR4 as significantly upregulated key biomarkers associated with cellular senescence and demethylation in acute myocardial infarction.

synapsesocial.com/papers/6a0c71a793c2b42b5c8869d1https://doi.org/10.1038/s41598-025-25391-9
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