A functional minigenome system revealed that swine orthopneumovirus RNA polymerase activity depends on a conserved PP1 binding site, as an F131A substitution markedly reduced polymerase function.
The development of a functional minigenome system for swine orthopneumovirus reveals key polymerase features, paving the way for reverse genetics and pathogenicity studies.
Swine orthopneumovirus (SOV), a recently identified porcine pneumovirus, has been detected in pig farms worldwide; however, its pathogenicity and molecular biology remain poorly understood. To facilitate the study of SOV replication and transcription, we developed a functional minigenome system based on consensus sequences from multiple strains of SOV and related pneumoviruses. Here, we constructed and optimized this system in BSRT7/5 cells, revealing that the RNA-dependent RNA polymerase (RdRp) activity depends on a conserved protein phosphatase 1 (PP1) binding site within the phosphoprotein P, as a single F131A substitution markedly reduced polymerase function. Additionally, we identified and characterized the M2-1 binding site on P, which is essential for viral transcription. These findings provide new insights into SOV polymerase complex requirements and establish a foundation for reverse genetics approaches to rescue infectious viruses, advancing our understanding of SOV biology and its potential role in porcine respiratory disease.IMPORTANCERecently, a newly identified porcine pneumovirus, swine orthopneumovirus (SOV), was detected in pig farms in different countries. Although detected mainly in sick animals, this virus has not been isolated yet and its pathogenicity remains to be determined. We started by setting up a minigenome system with a view to develop reverse genetics and rescue infectious virions. This minigenome system was used to study the functioning of the SOV RNA polymerase and compared it with RSV. Although some similarities exist between SOV and RSV, the RdRp of RSV cannot rescue the SOV minigenome. SOV seems to belong to another genus/genogroup of pneumoviruses, which includes PVM and the canine pneumovirus. Our functional minigenome paves the way for reverse genetics of SOV and determination of its pathogenicity in different host species.
Richard et al. (Tue,) conducted a other in Swine orthopneumovirus (SOV). Functional minigenome system vs. RSV was evaluated on RNA-dependent RNA polymerase (RdRp) activity. A functional minigenome system revealed that swine orthopneumovirus RNA polymerase activity depends on a conserved PP1 binding site, as an F131A substitution markedly reduced polymerase function.
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