A chemiluminescent microfluidic thrombin generation assay for real-time monitoring in patient plasma

BACKGROUND: The thrombin generation assay (TGA) is a global coagulation test able to determine the amount of thrombin formed in plasma samples. TGA has been shown to be useful to monitor the effect of procoagulant and anticoagulation therapies in patients with bleeding diatheses or an increased thrombosis risk. OBJECTIVES: ). METHODS: . By only applying 20 μL of citrated plasma to the microfluidic cartridges, thrombin generation was monitored successfully with the characteristic peak profile in 110 nL detection chambers using a prototype readout device. RESULTS: was able to detect the defect in the plasma of patients with hemophilia A (pwHA), as well as the hemostatic replacement effect of factor VIII infusion in pwHA. CONCLUSION: is applicable for monitoring therapy of pwHA at a global level and monitoring anticoagulant therapy with DOACs using a portable readout device.