May 1, 1987Open Access
Effect of internal viral sequences on the utility of retroviral vectors
PICO estructurado
P
Población
Moloney murine leukemia virus-based vector expressing human ADA cDNA
I
Intervención
Inclusion of intron-contained sequences and viral gag gene sequences
O
Resultado
Virus titer generation and cDNA expressionsurrogate
Inclusion of viral gag gene sequences in Moloney murine leukemia virus-based vectors significantly increases viral titers, improving retroviral vector utility.
Resumen
Expression of the human ADA cDNA encoded by the Moloney murine leukemia virus spliced RNA form is enhanced by intron-contained sequences. The presence of sequences corresponding to the viral gag gene in a Moloney murine leukemia virus-based vector results in the generation of 10- to 40-fold higher titers of virus.