Glucose turnover values in the dog obtained with various species of labeled glucose

Recent studies proposed that 2Tglucose is preferable to 14C-glucose as a tracer for the measurement of glucose turnover. However, higher values for glucose turnover were obtained using 2Tglucose than with 14Cglucose. The present study explores the merit of another species of tritiated glucose, 3Tglucose. Utilizing isotope-dilution principles, comparison is made of glucose turnover values determined by use of 2Tglucose, 3Tglucose, and 6-14Cglucose. Glucose turnover using 2Tglucose was 1.51 +/- 0.07 times greater than that using 6-14Cglucose, after correction for recycling of 14C. However, glucose turnover values obtained with 3Tglucose were similar to those obtained with 6-14Cglucose. There were no temporal or quantitative differences in appearance of tritium (T) in plasma water after injection of 2T- and 3Tglucose. A methylprednisolone regimen in the normal dog increased glucose turnover as determined by all three tracers, but the increase observed using 2Tglusoce was significantly greater than that using that two other tracers. Thiement for 6-14Cglucose for measurement of glucose turnover in the dog.