Mechanical circulatory support in heart failure patients was associated with significantly elevated IL-1B mRNA expression in left (2.0-fold, p=0.0058) and right (3.3-fold, p<0.0001) ventricles.
Observational (n=63)
Does mechanical circulatory support alter the mRNA expression of Interleukin-1 receptors and related cytokines in myocardial tissue of patients with end-stage heart failure?
Mechanical circulatory support in end-stage heart failure is associated with enhanced local IL-1β signaling and altered IL-1 receptor expression in myocardial tissue.
valor p: p=0.0058 (LV), <0.0001 (RV)
Serum levels of cytokines interleukin 1 beta ( IL-1β) and interleukin 33 (IL-33) are highly abnormal in heart failure and remain elevated after mechanical circulatory support (MCS). However, local cytokine signaling induction remains elusive. Left (LV) and right ventricular (RV) myocardial tissue specimens of end-stage heart failure (HF) patients without (n = 24) and with MCS (n = 39; 594 ± 57 days) were analyzed for cytokine mRNA expression level of IL-1B, interleukin 1 receptor 1/2 (IL-1R1/2), interleukin 1 receptor-like 1 (IL-1RL1), IL-33 and interleukin-1 receptor accessory protein (IL-1RaP). MCS patients showed significantly elevated IL-1B expression levels (LV: 2.0 fold, p = 0.0058; RV: 3.3 fold, p < 0.0001). Moreover, IL-1R1, IL-1RaP and IL-33 expression levels strongly correlated with each other. IL-1RL1 and IL-1R2 expression levels were significantly higher in RV myocardial tissue (RV/LV ratio IL-1R2 HF: 4.400 ± 1.359; MCS: 4.657 ± 0.655; IL-1RL1 HF: 3.697 ± 0.876; MCS: 4.529 ± 0.5839). In addition, IL1-RaP and IL-33 RV expression levels were significantly elevated in MCS. Furthermore, IL-33 expression correlates with C-reactive protein (CRP) plasma levels in HF, but not in MCS patients. Increased expression of IL-1B and altered correlation patterns of IL-1 receptors indicate enhanced IL-1β signaling in MCS patients. Correlation of IL-1 receptor expression with IL-33 may hint towards a link between both pathways. Moreover, diverging expression in LV and RV suggests specific regulation of local cytokine signaling.
Niazy et al. (Fri,) conducted a observational in End-stage heart failure (n=63). Mechanical circulatory support (MCS) vs. Heart failure without MCS was evaluated on Cytokine mRNA expression levels of IL-1B, IL-1R1/2, IL-1RL1, IL-33 and IL-1RaP in myocardial tissue (p=0.0058 (LV), <0.0001 (RV)). Mechanical circulatory support in heart failure patients was associated with significantly elevated IL-1B mRNA expression in left (2.0-fold, p=0.0058) and right (3.3-fold, p<0.0001) ventricles.