Uses and Limitations of the XTT Assay in Studies of Candida Growth and Metabolism

Colorimetric tetrazolium assays are used increasingly in studies of fungi, often in the absence of standardization or correlation with other methods. We examined species- and strain-related tetrazolium metabolism in Candida albicans and Candida parapsilosis by using XTT 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl-2H-tetrazolium hydroxide] and WST-8 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphonyl)-2H-tetrazolium and found marked variations. Also, significant signal was often missed in the absence of dimethyl sulfoxide extraction.