MDM2 and CDK4 immunostainings demonstrated high sensitivity (97% and 92%) and specificity (83% and 95%) for identifying ALT-WDLPS and DDLPS among other soft tissue tumors.
Observational (n=559)
Are MDM2 and CDK4 immunostainings useful adjuncts in diagnosing well-differentiated and dedifferentiated liposarcoma subtypes?
MDM2 and CDK4 immunostainings are highly sensitive and specific adjuncts for differentiating ALT-WDLPS and DDLPS from benign adipose tumors and poorly differentiated sarcomas.
Atypical lipomatous tumor/well-differentiated liposarcoma (ALT-WDLPS) and dedifferentiated liposarcoma (DDLPS) may be difficult to distinguish from benign adipose tumors and from poorly differentiated sarcomas, respectively. Genetically, they are characterized by amplification of MDM2 and CDK4 genes on chromosome 12q13-15. We examined a series of 559 soft tissue tumors (44 ALT-WDLPS, 61 DDLPS, 49 benign adipose tumors, and 405 non-ALT-WDLPS/DDLPS sarcomas) for MDM2 and CDK4 expression using immunohistochemistry. MDM2 and CDK4 immunoexpressions were compared with gene amplification status (as assessed by quantitative PCR and/or comparative genomic hybridization) in 241 neoplasms. Most ALT-WDLPS/DDLPS expressed MDM2 (97%) and CDK4 (92%) as opposed to few benign adipose tumors (MDM2, 5%; CDK4, 2%) and a limited number of non-ALT-WDLSP/DDLPS sarcomas (MDM2, 19%; CDK4, 6%). The sensitivity and specificity of MDM2 and CDK4 immunostainings in identifying ALT-WDLPS/DDLPS among other soft tissue tumors were 97% and 92%, and 83% and 95%, respectively. MDM2 and CDK4 immunostainings were particularly useful to separate ALT-WDLPS from the large group of differentiated adipose tumors, and to distinguish DDLPS from poorly differentiated sarcomas. A strong correlation was observed between MDM2 and CDK4 stainings and gene amplification status. In conclusion, MDM2 and CDK4 immunostainings, which correlate with gene amplification, are helpful adjuncts to differentiate ALT-WDLPS from benign adipose tumors and to separate DDLPS from poorly differentiated sarcomas.
Binh et al. (Tue,) conducted a observational in Soft tissue tumors (ALT-WDLPS, DDLPS, benign adipose tumors, and other sarcomas) (n=559). MDM2 and CDK4 immunostaining vs. Benign adipose tumors and poorly differentiated sarcomas was evaluated on Sensitivity and specificity of MDM2 and CDK4 immunostainings in identifying ALT-WDLPS/DDLPS. MDM2 and CDK4 immunostainings demonstrated high sensitivity (97% and 92%) and specificity (83% and 95%) for identifying ALT-WDLPS and DDLPS among other soft tissue tumors.