Enzymatically controlled release of proteins and peptides: A promising, alternative secretion approach

expression at the cell surface and protease-mediated release, followed by collection from the cell culture medium. Using ENCOREP, we achieved the production of glycosylated peptides. These glycopeptides, derived from the large extracellular loop of the highly glycosylated CD63, illustrate the type of targets that are otherwise inaccessible with current methods but can be readily obtained using ENCOREP. Overall, ENCOREP provides a rapid and reliable approach to obtain glycosylated proteins or peptides while bypassing the conventional signal peptide-dependent secretory pathway.