Inhibition by lomofungin of nucleic acid and protein synthesis in Saccharomyces cerevisiae

,The phenazine an,~biotic lomofungin 1 inhibits the ~owth of many organisms 2 and it has been suggested that ~2ts primary ~ode of action ,~s on the synthesis of RNA 2. In t~e present ~nvest~gat,5on we have stud~ed the effect of the dnag an nucleic acid rnd protein symhesis in spheroplasts of Sacckaromyce~ cere~siae. Spheropiasts can carry out rapid synthesis of RNA and protein I3] and s~nce ~hey can be ly~d in det.ergent eas~y and qnieMy this has aEowed us to study, by sucrose gradient analysis, p@ribosome profiles before and aftei treatment of spheropIasls with the drug.