The present study focuses on the preparation and evaluation of an anti-inflammatory herbal spray using Bryophyllum pinnatum leaf extract for topical drug delivery. The plant is well known for its medicinal properties such as anti-inflammatory, analgesic, antimicrobial, and wound healing activities. Leaves were collected, authenticated, dried, and subjected to ethanolic extraction using the maceration method. The extract was further evaluated through phytochemical screening, confirming the presence of alkaloids, flavonoids, and tannins. The herbal spray was formulated using suitable excipients like Carbopol 940, propylene glycol, glycerin, and preservatives. The prepared formulation was evaluated for physicochemical parameters such as pH, viscosity, drying time, and organoleptic properties, which showed satisfactory results. The pH of the formulation was found to be skin-compatible. In- vitro anti-inflammatory activity was assessed using the protein denaturation method, and antibacterial activity was evaluated against E. coli, showing significant inhibition compared to the control. UV spectroscopic analysis confirmed the presence of active constituents in the formulation. The results indicate that the formulated Bryophyllum pinnatum herbal spray is effective, stable, and suitable for topical application. It offers a natural, safe, and cost-effective alternative to synthetic anti-inflammatory agents, with reduced side effects and good therapeutic potential.
Sakshi Patil1*, Rameshwari Ingavale2, Siddhesh Desai3, Shreyas Jadhav4, Kousalya A. Patil5 (Mon,) studied this question.