What does this research mean for the field?

The newly developed HKOH-2 series of fluorescent probes enables the selective, direct detection and imaging of highly reactive hydroxyl radicals in living cells, tissues, and in vivo with organelle-level precision. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research aims to develop fluorescent probes for the direct detection of hydroxyl radicals in living cells and tissues.

May 31, 2026

HKOH ‐2 Series Probes for Imaging Hydroxyl Radical in Living Cells With Subcellular Precision and In Vivo

Puntos clave

This research aims to develop fluorescent probes for the direct detection of hydroxyl radicals in living cells and tissues.
Developed HKOH‐2 series fluorescent probes for detection of hydroxyl radicals (• OH) in living cells, rat brain slices, and zebrafish.
Created HKOH‐2L for lysosomal detection and HKOH‐2m for mitochondrial detection of • OH.
Demonstrated successful imaging of hydroxyl radicals in living cells, with organelle-specific probes achieving subcellular resolution.
HKOH‐2 series provides versatile tools for monitoring local • OH production.

Resumen

ABSTRACT Hydroxyl radical ( • OH) is the most reactive and deleterious reactive oxygen species, yet its direct detection remains highly challenging because of its extremely short lifetime and high reactivity. Here we report the HKOH‐2 series of fluorescent probes for imaging • OH in living cells, rat brain slices, and living zebrafish. In addition, HKOH‐2L and HKOH‐2m were developed for selective detection of • OH with organelle resolution in lysosomes and mitochondria, respectively. The HKOH‐2 series thus provides versatile chemical tools for monitoring local • OH production in living cells and in vivo.

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