In situ cryopreservation within multi-well plates is a promising strategy for parallel biospecimen storage, yet it is critically limited by the lack of rapid and uniform rewarming techniques. This study presents an integrated electromagnetic induction heating system designed to address this bottleneck. A novel multi-well plate was developed, featuring integrated heaters that serve as internal heating elements upon immersion in the sample solution. Through systematic investigation and optimization of key parameters—including heater position, coil geometry and size, plate architecture, and the implementation of a field shaper—the system achieved a maximum rewarming rate of 762.4 °C/min while maintaining a maximum temperature difference below 6 °C across the optimized multi-well configuration. This performance represents a 25-fold increase in rewarming rate compared to conventional hotplate methods. The findings provide a scientific basis for parameter selection in induction-based rewarming and establish an efficient and reliable plate-scale rewarming strategy. This technology provides a critical thermal engineering framework and hardware foundation for future in situ preservation studies.
Han et al. (Fri,) studied this question.