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June 1, 2005Open Access

Genomic DNA Amplification from a Single Bacterium

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Resumen

Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying non-culturable microorganisms.

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Genomic DNA Amplification from a Single Bacterium | Synapse

Cite This Study

Raghunathan et al. (Wed,) studied this question.

synapsesocial.com/papers/6a20817d5654e44f693e88d0 https://doi.org/https://doi.org/10.1128/aem.71.6.3342-3347.2005

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5Isothermal Strand-Displacement Amplification Applications for High-Throughput Genomics2002 · 92 citations