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Cardiac mitochondria were prepared from male Sprague-Dawley rats weighing approximately 300 g which had been provided with rat chow and water ad libitum prior to killing.Subsarcolemmal mitochondria were separated from interfibrillar mitochondria by the procedure described by Palmer et al. (24) using slightly modified media.The 3 mM 4-morpholinopropanesulfonic acid buffer was replaced by 6742
Palmer et al. (Wed,) studied this question.