ATP directly increased junctional conductance in paired guinea pig ventricular myocytes in a dose-dependent manner, with a half-maximum effective concentration of 0.68 mM.
ATP directly regulates junctional conductance in ventricular myocytes, likely through a specific ligand-receptor interaction with gap junctional channel proteins.
Effects of ATP on junctional conductance (gj) were investigated in paired ventricular myocytes isolated from guinea pig hearts. One cell of the pair was voltage-clamped with a single-patch pipette, and gj was measured after the perforation of the nonjunctional membrane of the partner cell. The current-voltage relation of gj was linear between -30 and +30 mV. The control gj at 5.0 mM ATP in 88 pairs of cells ranged from 100 to 1,055 nS (average, 268 nS). ATP within the range from 0.1 to 5.0 mM increased gj in a dose-dependent manner. The Hill coefficient was 2.6, and the half-maximum effective concentration of ATP was 0.68 mM. Adenylylimidodiphosphate (2 mM) caused a transient increase in gj in the presence of 0.5 mM ATP, but forskolin (30 microM), cyclic AMP (50 microM), catalytic subunit of cyclic AMP-dependent protein kinase (1 microM), and ADP (10 mM) had no significant effect on gj. The temperature coefficient of gj in the presence of 5.0 mM ATP was 1.29. These findings suggest that gj in paired ventricular myocytes is directly regulated by ATP probably through a specific ligand-receptor interaction between ATP and gap junctional channel protein.
Sugiura et al. (Sun,) conducted a other in None (guinea pig ventricular myocytes) (n=88). ATP was evaluated on Junctional conductance (gj). ATP directly increased junctional conductance in paired guinea pig ventricular myocytes in a dose-dependent manner, with a half-maximum effective concentration of 0.68 mM.