Overexpression of A1 adenosine receptors in mouse hearts reduced post-ischemic necrosis by approximately 80% and selectively modified post-ischemic gene expression.
A1 adenosine receptor overexpression in mouse hearts provides cardioprotection against ischemia-reperfusion injury and selectively modifies post-ischemic gene expression.
OBJECTIVES: To identify potential molecular genetic determinants of cardiovascular ischemic tolerance in wild-type and transgenic hearts overexpressing A(1) adenosine receptors (A(1)ARs). METHODS: cDNA microarrays were used to explore expression of 1824 genes in wild-type hearts and ischemia-tolerant mouse hearts overexpressing A(1)ARs. RESULTS: Overexpression of A(1)ARs reduced post-ischemic contractile dysfunction, limited arrhythmogenesis, and reduced necrosis by approximately 80% in hearts subjected to 30 min global ischemia 60 min reperfusion. Cardioprotection was abrogated by acute A(1)AR antagonism, and only a small number (19) of genes were modified by A(1)AR overexpression in normoxic hearts. Ischemia-reperfusion significantly altered expression of 75 genes in wild-type hearts (14 induced, 61 down-regulated), including genes for metabolic enzymes, structural/motility proteins, cell signaling proteins, defense/growth proteins, and regulators of transcription and translation. A(1)AR overexpression reversed the majority of gene down-regulation whereas gene induction was generally unaltered. Additionally, genes involved in cell defence, signaling and gene expression were selectively modified by ischemia in transgenic hearts (33 induced, 10 down-regulated), possibly contributing to the protected phenotype. Real-time PCR verified changes in nine selected genes, revealing concordance with array data. Transcription of the A(1)AR gene was also modestly reduced post-ischemia, consistent with impaired functional sensitivity to A(1)AR stimulation CONCLUSIONS: Data are presented regarding the early post-ischemic gene profile of intact heart. Reduced A(1)AR transcription is observed which may contribute to poor outcome from ischemia. A(1)AR overexpression selectively modifies post-ischemic gene expression, potentially contributing to ischemic-tolerance.
Kevin J. Ashton (Sat,) conducted a other in Cardiovascular ischemic tolerance. A1 adenosine receptor (A1AR) overexpression vs. Wild-type hearts was evaluated on Post-ischemic contractile dysfunction, arrhythmogenesis, necrosis, and gene expression. Overexpression of A1 adenosine receptors in mouse hearts reduced post-ischemic necrosis by approximately 80% and selectively modified post-ischemic gene expression.
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