Analysis of Biological and Immunological Activities in the Two Pools of Lh Released During Constant Infusion of Luteinizing Hormone-Releasing Hormone (Lhrh) in Men

In response to constant infusion of a submaximal dose of LHRH (0.2 microgram/min for 4 hours), luteinizing hormone (LH) was released in a biphasic pattern in 6 normal men. When the in vitro biologic activity of plasma LH was compared to the immunologic activity throughout the response to LHRH, the B/I ratio remained unchanged (3.6 +/- 0.5, mean +/- SE). Thus, in men the biologic activity of stored LH (acutely releasable) is not different from the presumably newly synthesized hormone that is released as the second pool during prolonged LHRH infusion.