Green tea which has its origin in China is an aromatic beverage derived the withering and oxidation of Camellia sinensis leaves. It has been reported to have a wide therapeutic purpose; thus, greatly consumed. This study investigated the effects of the administration of various concentration of green tea on the semen quality and oxidative stress enzymes of adult male Sprague Dawley rats. The study was designed in line with the protocol and guideline of the Institutional Animal Care and Use committee (IACUC). Twenty (20) 58-62 days old male albino Sprague Dawley rats (Rattus Norvegicus), divided into five (5) animals per group were used for the experiment. Group 4; the control group received only distilled water, while Group 1, 2 and 3 were given 2ml/kg, 4ml/kg and 6ml/kg of aqueous extract of green tea respectively for 26 days. The animals were euthanized, the caudal epididymis was excised for semen analysis while the testis was homogenized for oxidative stress enzyme (Glutathione peroxidase and Malondialdehyde) assay. GraphPad Prism (version 8.0.2) Sidak’s multiple comparison test was used to evaluate the differences in group means. The result from semen analysis. Consumption of green tea at 2ml/kg does not disrupt the histoarchitecture of the testicular tissue; there was mild degeneration at 4ml/kg and 6ml/kg. There was simultaneous increase in the level of Glutathione peroxidase with the increase in concentration of green tea. There was a progressive increase for Malondialdehyde corresponding to a progressive decrease in the concentration of green tea. Thus, green tea helps to improve the quality of semen, reduction of fat and protection of the testicular tissue against oxidative stress.
Eric et al. (Tue,) studied this question.