Genomic DNA was uprooted from the tissue samples of Labeo Calbasu and Labeo rohita two economically meaningful cyprinid fish species and these two Labeo Species were collected from freshwater resources in the Marathwada region of Maharashtra in India DNA was Extracted from both species using their fins and tail fins the isolated mitochondrial DNA was amplified using PCR. The COI sequences (602–655 bp) were amplified and revealed the typical nucleotide pattern found in teleost fish mitochondria. The A+T content (54.8–55.2%) was higher than the G+C content, with guanine (G) being the least frequent base (17.4–17.6% Intraspecific variation was minimal: 0.95% in variable sites in Labeo Rohita (n=15) and 0.63% in Labeo Calbasu (n=12) with all substitutions being synonymous and predominantly at the third codon position. Haplotype diversity (Hd) was moderate in both species (0.452 for Labeo rohita and 0.389 for Labeo calbasu) while nucleotide diversity (π) was low (0.0018 and 0.0015 respectively for both species). Within both species, there was low average Kimura 2-parameter (K2P) genetic distance; 0.31% in Labeo rohita and 0.28% in Labeo calbasu. Still, the distance between the two species was important advanced at 4.82. The significant difference fluently indicates that there is a distinct gap between the barcodes the Kimura 2- parameter (K2P) heritable distance between Labeo rohita and Labeo calbasu were both low with a normal of 0.31 and 0.28 singly. The results show that COI DNA barcoding works veritably well for rightly relating and separating Labeo rohita and Labeo calbasu this study gives us useful inheritable information that can help with accurate fish identification more and more sustainable fish husbandry and proper conservation of these two important species in Indian brackish gutters and lakes.
Rode et al. (Sat,) studied this question.