Selected metastable peak monitoring: A new, specific technique in quantitative gas chromatography mass spectrometry

Using the combined electrostatic analyser-magnet scan facility of a double focusing mass spectrometer, the daughter ion peak for the fragmentation, M-57+ leads to M-57-76+, of a 5alpha-dihydrotestosterone tert-butyl-dimethylsilyl ether was monitored during gas chromatography mass spectrometry. The detection limit was approximately 20 pg. The steroid was detected by this method in an extract of human blood plasma.