The nucleotide sequence of a small (3S) seryl-tRNA (anticodon GCU) from beef heart mitochondria

The primary structure of a 3S serine tRNA from beef heart mitochondria has been determined using our new two-dimensional "read-off" sequencing method (Tanaka, Y., Dyer, T.A. and Brownlee, G.G (1980) Nucleic Acids Res. 8, 1259-1272). When arranged in the "cloverleaf" form it shows unique features since (i) it completely lacks the dihydrouridine arm, (ii) it has an extended "T gamma" loop, but lacks the T and gamma residues, and (iii) it has only one minor base, N6(N-threonylcarbamoyl)adenosine, next to the anticodon.