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September 1, 1958Biochemical Journal75 citationsOpen Access

A sensitive method for estimation of oxaloacetate

GKGeorge KalnitskyDTDonald F. Tapley

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Abstract

The coupling of acetoacetate with an excess of diazotized p-nitroaniline has been utilized by Walker (1954) to develop a sensitive colorimetric method for the estimation of acetoacetate. With defined conditions at pH 49-5*0, the reaction proceeds as in equation 1 (R=p-NO2.0CH4) with the formation of NN'-bis-(p-nitrophenyl)-C-acetylformazan.

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Kalnitsky et al. (1958) studied this question.

synapsesocial.com/papers/6a21a19ef6aa648d3a583815https://doi.org/10.1042/bj0700028
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