Eltanexor reduces COX-2 expression. A and B, Western blot probing for COX-2 protein in HCA-7 cells treated with either control or 200 nmol/L eltanexor for 48 hours. Actin was used as the loading control. The graph depicts the quantified densitometry of the Western blot bands. The values have been normalized to the control-treated cells. The graph shows the mean of three independent experiments ± SEM. A Student t test was used to statistically compare COX-2 expression in the control-treated and eltanexor-treated groups. C, COX-2 mRNA expression by qPCR in HCA-7 cells treated with DMSO control or 200 nmol/L eltanexor for 48 hours. The values are the mean of four experiments ± SEM. A Student t test was used to statistically compare COX-2 expression in the control-treated and eltanexor-treated groups. D, HCT116 cells transfected with a plasmid containing the COX-2 promoter + luciferase gene. The cells were subsequently treated with 200 nmol/L eltanexor for 48 hours. The graph shows the mean eltanexor-treated luciferase activity/μg of protein normalized to DMSO-treated values of three independent experiments ± SEM. A Student t test was used to statistically compare luciferase activity in the control-treated and eltanexor-treated groups. E, HCT116 cells were transfected with COX-2 promoter + luciferase gene. The promoter region was deleted from full-length down to 300 base pairs. Cells were subsequently treated with DMSO (gray bars) or 200 nmol/L eltanexor (red bars) for 24 hours. The graph shows the mean luciferase activity/μg of protein of eltanexor-treated cells normalized to DMSO-treated values of three independent experiments ± SEM. A Student t test was used to statistically compare luciferase activity in the control-treated and eltanexor-treated groups. F, Schematic representation showing the site of COX-2 expression promotion by Wnt/β-catenin signaling. G and H, Western blot probing for c-myc in HCT116 cells treated with either control or 200 nmol/L eltanexor for 48 hours. The graph depicts the mean of c-myc band densitometry value normalized to actin of three independent experiments ± SEM. A Student t test was used to statistically compare c-myc expression in control-treated and eltanexor-treated groups. (*, P ≤ 0.05; **, P ≤ 0.01.). NS, not significant.
Evans et al. (Tue,) studied this question.