Mentha piperita L. (peppermint) is a valued medicinal and industrial plant known for its essential oils and bioactive compounds, particularly menthol. This study investigates the effects of four phytohormonal elicitors, methyl jasmonate (MJ), abscisic acid (ABA), salicylic acid (SA), and gibberellic acid (GA) on oxidative stress, gene expression, and the biosynthesis of menthol and related monoterpenes in peppermint. Treatments at two concentrations, 0.2 mM and 0.4 mM, were evaluated using quantitative real-time PCR (qRT-PCR) for gene expression and gas chromatography–mass spectrometry (GC-MS) for metabolite profiling. The results showed that MJ and SA at 0.4 mM significantly enhanced menthol and menthone levels while reducing menthofuran accumulation. ABA reduced menthol content at the same concentration, whereas GA had a limited impact on metabolite levels. Expression of key genes associated with the menthol biosynthetic pathway, including the biosynthetic genes PR, MDH, and MFS, as well as the regulatory transcription factors MhMYB1 and MhMYB2, was significantly affected by the treatments. MJ and SA particularly upregulated MhMYB1, MhMYB2, PR, and MDH. All phytohormones induced oxidative stress and elevated antioxidant enzyme activities, with MJ and ABA showing the strongest effects. The findings highlight the diverse regulatory roles of phytohormones in menthol biosynthesis. These results suggest that MJ and SA can be used to optimize menthol production in peppermint.
Khaldari et al. (Wed,) studied this question.
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