What type of study is this?

This is a Quantitative Study study (also classified as: Animal Study).

September 29, 2025

Abstract B011: Functional interrogation of the pancreatic intratumoral microbial niche reveals tumor modulation and reshaping of the microenvironment

Key Points

Findings show microbial regulation alters signaling pathways in pancreatic cancer cells, influencing immune evasion.
Spatial transcriptomics revealed the subcellular location and function of microbes within pancreatic tumors, showcasing their roles.
The study utilized quantitative fluorescent imaging techniques and in vitro co-culture assays to validate results.
These results highlight the promise of the microbial niche as a target for therapeutic intervention in pancreatic cancer.

Abstract

Abstract Tumor resident microbes are a well-recognized component of the tumor microenvironment. Microbial subcellular location within different tumor types along with their functionality remains to be determined. Bulk microbial profiling techniques, however, lack subcellular and spatial transcriptomic resolution. To address these limitations, we developed advanced quantitative fluorescent imaging methodologies that allowed for visualization of microbial cellular compartmentalization across different tumor types. Furthermore, we performed spatial microbial transcriptomics at the regional and single cell levels to interrogate microbial regulation of signaling within pancreatic cancer cells. To confirm presence of viable microbes within tumors, we performed multiplexed culturomics of patient tissue samples followed by Whole Genomic Sequencing (WGS) analysis. We used in vitro co-culture assays together with in vivo animal experiments for functional biological validation. Overall, our results identified several pathways under microbial regulation within cancer cells that can drive immune evasion through impaired antigen presentation. In summary, using multiple complimentary novel methodologies we characterize the microbial niche of tumors (MiNT) that uncovers microbial regulation of host cells signaling and reshaping of the surrounding tumor microenvironment. Citation Format: Vidhi Chandra, Le Li, Seyda Baydogan, Haoyue Liu, Javier Gomez, Steven B. Maron, Daniel Carvajal-Hausdorf, Erick M. Riquelme, Mark W. Hurd, Anirban Maitra, Luis Diaz, Ismet Sahin, Jared Burks, Michael P. Kim, Florencia McAllister. Functional interrogation of the pancreatic intratumoral microbial niche reveals tumor modulation and reshaping of the microenvironment abstract. In: Proceedings of the AACR Special Conference in Cancer Research: Advances in Pancreatic Cancer Research—Emerging Science Driving Transformative Solutions; Boston, MA; 2025 Sep 28-Oct 1; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2025;85 (18Suppl₃): Abstract nr B011.

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Cite This Study

Chandra et al. (Sun,) studied this question.

synapsesocial.com/papers/68da58dcc1728099cfd11365 https://doi.org/https://doi.org/10.1158/1538-7445.pancreatic25-b011

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