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Introduction: Looking for novel amylases needed for industrial processes may benefit from the screening of microorganisms with high amylase activity. Microbial enzymes are widely used in industrial processes due to affordable cost, significant production, chemical stability, environmental protection, plasticity, and widespread availability. Since Purba Bardhaman contains numerous rice processing plants and agro-industrial waste disposal locations, water samples were gathered for the study. Material & Methods: Amylase-producing bacteria were isolated, identified and revealed from this work to add new knowledge into the field of science. Results: Seven bacterial isolates were isolated, and based on appearance of zone of hydrolysis in starch agar plates was selected for further study. The isolate was Gram-positive, spore-forming rods. Depending on 16s rRNA gene sequences with 99.79% similarity the isolate was identified as Brevibacillus parabrevis, efficient amylase producers with an excellent yield throughout the solid-state fermentation technique. The isolate demonstrated maximum enzyme activity of 350 U/mg at 5% substrate concentrations. This piece of work focuses on finding out whether agro-industrial waste water could be a better source for amylolytic bacteria.
Soumya Nandi (Tue,) studied this question.