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A Gram-stain-positive bacterium, designated YN-L-19 T , was isolated from a sludge sample collected from a pesticide-manufacturing plant. Cells of YN-L-19 T were strictly aerobic, non-spore-forming, non-motile and ovoid-shaped. Colonies were small, smooth and yellow. Growth occurred at 10–37 °C (optimum, 30 °C), pH 5.0–9.0 (optimum, 7.0) and 0–3.0 % (w/v) NaCl (optimum 0.5 %). Phylogenetic analysis based on genome and 16S rRNA gene sequences indicated that YN-L-19 T was affiliated to the family Microbacteriaceae and most closely related to Diaminobutyricimonas aenilata , Terrimesophilobacter mesophilus , Planctomonas deserti and Curtobacterium luteum . The major cellular fatty acids of YN-L-19 T were anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 16 : 0 and C 16 : 0 . The predominant menaquinone was MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unidentified lipid. The average amino acid identity values between strain YN-L-19 T and the related strains were 57.9–61.9 %, which were below the genus boundary (70 %). On the basis of the evidence presented in this study, strain YN-L-19 T represents a novel species of a new genus in the family Microbacteriaceae , for which the name Ruicaihuangia caeni gen. nov., sp. nov. (type strain YN-L-19 T =CCTCC AB 2022401 T = KCTC 49935 T ) is proposed.
Liu et al. (Tue,) studied this question.