Complete Genome Sequence Analysis of the Bacterial Wilt Disease Pathogen Ralstonia pseudosolanacearum Strain MLY158

Background: The Ralstonia solanacearum species complex (RSSC) is a group of destructive plant-pathogenic bacteria that targets a wide range of economically important crops across the globe, including tomato, pepper, and tobacco. Extensive research on this plant pathogen is essential due to the severe losses it inflicts on agricultural production. Methods: We isolated strain MLY158 from diseased tobacco, identifying it as Ralstonia pseudosolanacearum. The strain was characterized genomically by biochemical profiling, genome sequencing, compositional and functional annotation, and comparative genomics. Results: MLY158 was capable of utilizing D-glucose (dGLU), sucrose (SAC), and D-trehalose dihydrate (dTRE). The genome had a total size of 5.88 MB and consisted of a circular chromosome and a circular megaplasmid. It contained 5485 coding genes and had a GC content of 67.50%. Comparative genomic results revealed that MLY158 is closely related to R. pseudosolanacearum strain GMI1000 (average nucleotide identity (ANI) value of 99.03%). MLY158 has 527 special genes and 13 homologous genes of species-specific gene families. The primary differences between MLY158 and genomes from other strains are located in the phage protein region and show characteristics of high genomic uniqueness. Conclusions: Complete genome sequence analysis of MLY158 has contributed important information regarding the genome of the bacterial wilt disease pathogen R. pseudosolanacearum. This work provides useful references for future research into molecular disease control strategies and disease-resistant breeding.