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Objective: A validated Hydrophilic Interaction Liquid Chromatography (HILIC) method was developed for the quantitative determination of Favipiravir. Methods: The chromatographic analysis was performed using a high-performance liquid chromatography (HPLC) system comprising a diode array detector, vacuum pump, column thermostat, and autosampler (Agilent 1260, Agilent Technologies, Santa Clara, CA, USA). The analysis was conducted on a ZORBAX RRHD HILIC Plus analytical column (95 Å, 2.1 × 150 mm, 1.8 µm). The mobile phase consisted of acetonitrile and water (80:20, v/v). Chromatographic separation was achieved under isocratic elution at a flow rate of 0.8 mL/min, and Favipiravir was detected at a wavelength of 322 nm. Results: The retention time of Favipiravir was 1.8317 ± 0.002 min, with a limit of detection (LOD) of 0.14 μg/mL and a limit of quantification (LOQ) of 0.47 μg/mL. The regression equation was y = 107.54x + 17.034 with a correlation coefficient (r²) of 0.9997. Conclusion: The proposed method was validated in terms of specificity, linearity, accuracy, precision, LOD, and LOQ, and was successfully applied for the quantitative analysis of Favipiravir in tablet formulations.
Dilhe Nur Çim (Wed,) studied this question.