What question did this study set out to answer?

This research aims to identify the conformational variations of alpha-synuclein in different solutions.

February 19, 2026The Analyst0 citationsOpen Access

Differences in α-synuclein conformational states in physiologically relevant pH/Na + concentrations and ammonium acetate solutions unveiled by native mass spectrometry

EBErick G. Báez BolívarPurdue University West Lafayette JFJessica Fortin TATaiwo A. AdemoyePurdue University West Lafayette

Key Points

This research aims to identify the conformational variations of alpha-synuclein in different solutions.
Utilized native mass spectrometry with theta emitters
Compared wild-type and mutant alpha-synuclein
Performed analyses at physiological pH and sodium concentrations
Included ammonium acetate solutions for comparison
Demonstrated distinct conformational states between wild-type and mutant alpha-synuclein
Observed variations based on pH and sodium ion concentrations
Highlighted the effect of ammonium acetate on protein conformation

Abstract

Native MS implemented with theta emitters was used to demonstrate differences in conformational states of wild-type and mutant α-synuclein dissolved at physiologically relevant pH and Na + concentrations compared to solutions of ammonium acetate.

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