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This research aims to explore how microsomal rafts affect DNA import into potato mitochondria.

February 20, 2026Биологические мембраны / Membrane and Cell Biology0 citations

Influence of Microsomal Rafts on DNA Import into Mitochondria of Potato Tubers (Solanum tuberosum L.)

Key Points

This research aims to explore how microsomal rafts affect DNA import into potato mitochondria.
Isolation of microsomes using ultracentrifugation and Triton X-100 treatment
Analysis of raft fractions in sucrose gradients
Assessment of DNA import activity in organello
Comparison of different raft fractions based on their membrane origin
Raft fractions enhanced DNA import activity between 4 to 23 times depending on their origin
Three distinct opalescence zones were observed in the sucrose gradients
Individual raft fractions varied in their effectiveness for DNA import into mitochondria

Abstract

The influence of microsomes and lipid rafts isolated from them on DNA import into potato (Solanum tuberosum L.) mitochondria was studied. Ultracentrifugation of microsomes treated with Triton X-100 revealed three opalescence zones containing rafts in 15%, 25%, and 35% sucrose gradients. When individual raft fractions were added to the mitochondrial DNA import system in organello, their ability to enhance DNA import activity to varying degrees (from 4 to 23 times, depending on their membrane origin) was established.

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