Intraspecific Polymorphism of Saccharomyces paradoxus Yeasts: Geographical Populations

In the Siberian Botanical Garden of Tomsk State University, 28 strains of Saccharomyces yeasts were isolated from the bark of Quercus robur and the soil beneath them. Based on the sequencing of the D1/D2 domain and the ITS1 region of rDNA, three strains were assigned to the species S. cerevisiae, and the rest to the species S. paradoxus. Using multigene phylogenetic analysis of nuclear genes (NEJ1, EST2, HDF1, HDF2), the genetic relationship of West Siberian strains of S. paradoxus with European, Far Eastern, North American and Hawaiian populations was studied. According to the results obtained, the West Siberian strains belong to the European population. Apparently, the border between the European and Far Eastern populations of S. paradoxus lies east of Tomsk Oblast.