What question did this study set out to answer?

The research aims to quantify bacterial load in the spleen to evaluate infection severity in mice.

February 28, 2026

Assessing Bacterial Burden in the Mouse Spleen for Infection Studies

Key Points

The research aims to quantify bacterial load in the spleen to evaluate infection severity in mice.
Non-invasive method used for induction of bacterial pneumonia in mice.
Spleens are homogenized and then serially diluted.
Diluted samples are plated to measure total bacterial burden.
Identified correlations between bacterial load and severity of infection.
Provided insights on the dynamics of host-pathogen interactions.

Abstract

Source: Madenspacher, J. H. and Fessler, M. B. A Non-invasive and Technically Non-intensive Method for Induction and Phenotyping of Experimental Bacterial Pneumonia in Mice. J. Vis. Exp. (2016)This video demonstrates the process of quantifying bacterial load in the spleen of an infected mouse to assess the severity of infection. The homogenized spleen is serially diluted and plated to determine total bacterial burden. The resulting data provide insights into host-pathogen interactions within systemic tissue.

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Cite This Study

TODO TODO (Thu,) studied this question.

synapsesocial.com/papers/69a286370a974eb0d3c00fd0 https://doi.org/https://doi.org/10.3791/24486

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