What question did this study set out to answer?

To model bacterial-fungal interactions in oral biofilms to understand their role in oral diseases.

February 28, 2026

Establishing Bacterial–Fungal Cross-Kingdom Biofilms for Studying Oral Pathogenesis

Key Points

To model bacterial-fungal interactions in oral biofilms to understand their role in oral diseases.
Cultivated Streptococcus mutans and Candida albicans together under oral-like conditions.
Used confocal microscopy to monitor biofilm formation and behavior.
Assessed microbial interactions and their impacts on virulence.
Successful formation of cross-kingdom biofilms was achieved.
Observations showed enhanced microbial synergy in the biofilm.
Acidogenic virulence indicators were observed in biofilm environments.

Abstract

Source: Schlafer, S, and Frost Kristensen, M. Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy. J. Vis. Exp. (2020)This video demonstrates the cultivation of Streptococcus mutans and Candida albicans cross-kingdom biofilms under oral-like conditions to model bacterial–fungal interactions, enabling the study of microbial synergy, glucan-mediated adhesion, and acidogenic virulence in oral pathogenesis.

Bookmark

Cite This Study

TODO TODO (Thu,) studied this question.

synapsesocial.com/papers/69a286370a974eb0d3c00fdd https://doi.org/https://doi.org/10.3791/24451

Bookmark