The brain is a complex structural network. The employment of fluorescent labeling techniques in conjunction with advanced imaging methodologies facilitates comprehensive analysis of multiscale brain anatomy, thereby offering insights into fundamental principles of function and addressing neurological disorders. This review summarizes technological advances in fluorescent labeling methods in the field of neuroscience, and their applications in neural circuit analysis, cerebrovascular imaging, neuronal activity monitoring, and fluorescence-guided treatment of brain tumors. A challenging trend in integrating smart fluorescent labeling with tissue clearing, wide-field 3D imaging, artificial intelligence-assisted data processing/reconstruction, and multimodal information fusion is highlighted and discussed. The future direction of combining high-resolution, low-damage, dynamic imaging with big data analysis is envisioned, providing tools for understanding brain structure and function and their roles in disease.
Yin et al. (Sat,) studied this question.