A Robust Methodological Framework for Generating Whole-Brain and Cortical Organoids From Diverse Healthy- and Patient-Derived Induced Pluripotent Stem Cell Lines.

Key Points

• The aim is to establish a reliable method for creating brain organoids from various induced pluripotent stem cell lines to study neurodevelopment and disease.
• Developed a comprehensive framework for organoid generation
• Utilized genetically diverse healthy and patient-derived hiPSC lines
• Focused on producing robust and reproducible organoids
• Successfully generated neuro-organoids from diverse hiPSC sources
• Demonstrated reliability in studying human neurodevelopment
• Enabled exploration of progeroid and disease-specific mechanisms