March 3, 2026

Acute LC50 Value of Methotrexate in Drosophila Melanogaster Larvae

Key Points

50% mortality was observed at 164.9 ppm methotrexate after 24 hours of acute exposure.
Probit analysis was used to assess the dose-response relationship in larvae exposed to various concentrations.
Samples included third instar larvae of Drosophila melanogaster to determine toxicity levels accurately.
Findings highlight the potential risks associated with methotrexate levels in biological systems.

Abstract

AbstractThe value of LC50 of the commercially available immunosuppressive drug methotrexate (MTX) was determined using the larvae of Drosophila melanogaster. For this purpose, third instar larvae were exposed to different concentrations of MTX (75, 100, 150, 200 and 250 ppm). Probit analysis was done on the dose-response data in order to find LC50. The results indicated that after 24 hours of acute exposure, larvae subjected to 164.9 ppm MTX showed 50% mortality. Hence the LC50 value was found to be 164.9 ppm for MTX in third instar larvae of Drosophila melanogaster.

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Cite This Study

Patel et al. (Wed,) studied this question.

synapsesocial.com/papers/69a75b90c6e9836116a230f6 https://doi.org/https://doi.org/10.5958/0976-4755.2025.00177.7

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