Research on the reproductive tract microbiology of broodmares has primarily focused on the uterus, with a limited set of tools for the rapid detection of pathogenic bacteria in the perineal region. Accurate, real-time identification of bacterial contamination could improve the diagnosis and management of post-breeding infectious endometritis. In this proof-of-concept study, we evaluated the ability of a portable MolecuLight i:X fluorescence imaging device for the rapid, non-invasive detection of potentially pathogenic perineal bacteria in healthy broodmares, comparing results with microbiological culture as the gold standard. Using ultraviolet-induced fluorescence imaging guided for swabbing and microbiological culture, the device demonstrated 80% sensitivity, 96% specificity, and 91% accuracy in differentiating potential pathogenic from commensal bacteria in clinically healthy broodmares. These preliminary findings may represent the basis for further assessment of the real-time, fluorescence-based technology in diseased or symptomatic broodmares, potentially aiding timely clinical decision-making. Further multicentred studies with larger inclusion of mares with confirmed endometritis are needed to strengthen the relevance of this technology and to expand the device’s application in equine reproductive health.
Bazzano et al. (Wed,) studied this question.