A combination of domain fusion and homologous site transplantation improves the performance of Bst DNA polymerase in denaturation bubble-mediated strand exchange amplification

Key Points

• Improvement in strand exchange amplification performance was observed with Bst DNA polymerase, indicating effective enhancement tactics.
• The study shows a 30% increase in amplification efficiency, underscoring the importance of innovative techniques in enzymatic functions.
• Assessment using novel domain fusion and homologous site transplantation provides new insights into enzyme performance.
• These results suggest domain modifications could lead to broader applications in biotechnology and molecular biology.