Reprogramming carbon metabolism and enhancing the Kennedy pathway promote docosahexaenoic acid biosynthesis in Schizochytrium sp. HX-308

Key Points

• Docosahexaenoic acid biosynthesis is significantly enhanced through carbon metabolism reprogramming and Kennedy pathway activation.
• Increased lipid synthesis was observed, leading to higher levels of docosahexaenoic acid in the microalgae strain.
• Metabolic engineering techniques were applied to optimize carbon metabolism and the Kennedy pathway in Schizochytrium sp.
• Findings suggest that these interventions may lead to more efficient production methods for valuable omega-3 fatty acids.