Design and construction of a novel transcription factor-based glycine biosensor for directed enzyme evolution in Escherichia coli

Key Points

• The biosensor effectively detects glycine, facilitating enzyme evolution in controlled conditions.
• Key evidence includes a demonstrated glycine detection limit of 5 µM under ideal conditions.
• Utilizing a transcription factor-based design, this innovative biosensor allows for real-time monitoring of glycine levels.
• This advancement in glycine detection may enable enhanced biochemical pathways in engineered Escherichia coli.