Introduction Cyathula capitata (Wall. ) Moq. , a widely used medicinal herb in Yi medicine, is often combined with other herbs to treat traumatic injuries, rheumatism, and rheumatoid arthritis. This study aims to characterize the chloroplast genomes, assess variation levels, and elucidate the phylogenetic relationships among Cyathula capitata, Cyathula officinalis, and their hybrid (Cyathula officinalis × Cyathula capitata). The findings provide valuable references for species identification, genetic background analysis, and quality control of Cyathula medicinal materials. Methods We employed second-generation high-throughput sequencing technology to sequence the chloroplast genomes of Cyathula capitata, Cyathula officinalis, and their hybrid (Cyathula officinalis × Cyathula capitata). Comparative genomic analyses were conducted to examine their genomic structures, simple sequence repeats (SSRs), codon usage preferences, and inverted repeat (IR) regions. Additionally, a phylogenetic tree based on chloroplast genome sequences was constructed to clarify their evolutionary relationships. Results The chloroplast genomes of all three taxa displayed the typical quadripartite structure, comprising a large single-copy region, a small single-copy region, and two IR regions (IRa and IRb). The Cyathula capitata genomes ranged from 151, 428 to 151, 436 bp, showing notable intraspecific diversity likely influenced by geographic factors. However, two C. capitata samples shared an identical genome length of 151, 518 bp and exhibited consistent genotypes across locations, indicating relative genomic conservation. Repeat sequence analysis identified hexanucleotide SSRs, a unique 16-bp single nucleotide insertion in the rpl22 gene, and a 30–40 bp forward repeat within the rps16₁-trnQ-UUG intergenic region as distinguishing markers for Cyathula officinalis and its hybrids. Codon usage analysis revealed no strong bias at the third codon position among the three species, although codons ending with thymine (T) were used more frequently. IR boundary analysis showed variation only among isolated C. capitata samples. Comparative genomics highlighted psbI-trnS-GCU and rps16₁-trnQ-UUG as highly variable regions. Phylogenetic analysis indicated that the hybrid (Z4) grouped within C. capitata, consistent with the maternal inheritance pattern of chloroplast genomes. Given the potential for variations in maternal parents among different hybrid batches, the positions of these elements in the phylogenetic tree may accordingly vary. Conclusion This study elucidated the chloroplast genome features and phylogenetic relationships of C. capitata, C. officinalis, and their hybrid. The findings offer significant molecular insights that facilitate species identification, genetic analysis, and quality assessment of Cyathula medicinal resources. These insights support the sustainable utilization and conservation of these resources.
Gao et al. (Wed,) studied this question.