Dual testing with immunohistochemistry and PCR for enhanced accuracy in dMMR/MSI detection in Greek colorectal cancer population: the impact of retesting

Key Points

• The study aims to evaluate the effectiveness of dual testing using immunohistochemistry and PCR for detecting dMMR/MSI in colorectal cancers.
• Conducted a retrospective analysis in a single center
• Performed dual assessment of MMR status using IHC and PCR
• Analyzed inter-method agreement and rates of misclassification
• Achieved near-perfect agreement between IHC and PCR methods
• Reduced likelihood of technical misclassification
• Supported the value of reflex retesting for quality assurance in diagnostics