Background: The present study explores the antibacterial property of a pineapple crown leaf extract (PCLE) and pineapple peel extract (PPE)-based novel chemomechanical caries removal (CMCR) agents against cariogenic bacteria. Materials and Methods: After initial screening of antibacterial property using well-diffusion method, CMCR agents containing 5% PCLE and PPE were used to test its antibacterial property in terms of cytoplasmic leakage, protein leakage, and nuclear leakage against Streptococcus mutans and Lactobacillus acidophilus . The results of all assays were expressed as optical density (OD) based on the absorbance at specific wavelength. Time kill curve assay was carried out to assess whether there was a time and dose-dependent antibacterial activity for the tested products. For all assays, papain-based CMCR agent was maintained as the standard control. Intergroup comparison of OD values was carried out using Kruskall–Wallis followed by post hoc analysis. Results: For S. mutans , cytoplasmic leakage and DNA leakage were higher for Test Group 1 (PCLE) when compared to Test group 2 (PPE) and standard control while protein leakage was higher for Test group 2 when compared to standard control. For L. acidophilus , protein leakage was higher for both the test groups when compared to standard control while DNA leakage was more for Test group 1 when compared to standard control. Time kill curve analysis showed that Test group 1 had better time kill. Conclusions: PCLE- and PPE-based CMCR agents have better antibacterial property than the standard control in terms of cytoplasmic leakage, protein leakage, DNA leakage, and time kill curve assay.
Sivasamy et al. (Thu,) studied this question.